ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]

About this product

ZIP Kinase is a serine/threonine kinase, which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca (2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Specifications

Catalog number

HA721777

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

DAP kinase 3 antibody DAP like kinase antibody DAP-like kinase antibody Dapk 3 antibody DAPK3 antibody DAPK3_HUMAN antibody Death associated kinase 3 antibody Death associated protein kinase 3 antibody Death-associated protein kinase 3 antibody Dlk antibody EC 2.7.11.1 antibody FLJ36473 antibody MYPT1 kinase antibody ZIP antibody ZIP kinase antibody ZIP kinase isoform antibody ZIP-kinase antibody ZIPK antibody zipper-interacting protein kinase antibody

Abbreviation

DAPK3, ZIPK

Swiss Prot

O43293 Human

Cellular Locus

Nucleus, Cytoplasm

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human ZIP Kinase aa 250-454 (O43293).

Isotype

IgG

Clone

PSH02-18

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB

Positive Control

A431 cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, Caco-2 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, human kidney tissue lysate.

Concentration

1 mg/mL

Dilution

WB: 1:1,000-1:2,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 53 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes