Adenosine Diphosphate (ADP) Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F009-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

ADP

Type

Fluorometric method

Field of Research

Glycolysis and Lipid Metabolism; Metabolic Diseases

Assay Principle

Adenosine diphosphate (ADP) is a nucleoside that plays a key role in energy transfer reactions. In organisms, ADP is usually the product of Adenosine triphosphate (ATP) which hydrolysis after the loss of a phosphate group, that is breaking a high-energy phosphate bond and releasing energy. ADP exists in high-density particles in platelet cells and is released when platelets undergo coagulation reaction. ADP affects the shape and biological behavior of platelets through ADP receptors on platelets, and further accelerates the process of platelet coagulation. The detection principle of this kit is that ADP and the substrate are catalyzed by enzyme reagents to make the chromogenic agent produce fluorescence. The fluorescence microplate reader was used to detect the excitation wavelength of 535 nm and the emission wavelength of 587nm, and the ADP content in the sample was calculated according to the standard curve.

Assay Performance Time

60 min

Sample Type

Serum; plasma; animal tissue; cell

Detection Range

2.8-50 μmol/L

Sensitivity

2.8 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

4.4-9.6%

Intra CV

4.0-5.0%

Detection Instrument

Fluorescence Microplate Reader