Proteinase K (lyophilized powder)

About this product

<span data-teams="true">Proteinase K is a highly active recombinant serine protease derived from Tritirachium album, widely used in nucleic acid extraction, molecular diagnostics, and biopharmaceutical sample preparation. It exhibits broad substrate specificity and efficiently digests proteins under both native and denaturing conditions, including in the presence of detergents such as SDS and urea.<br>Developed through directed evolution technology, Proteinase K delivers enhanced enzymatic activity, exceptional stability, and excellent compatibility with nucleic acid purification workflows. The product is free of detectable DNase, RNase, and nickase contamination, ensuring reliable performance in sensitive DNA and RNA applications.</span>

Specifications

Catalog number

HH4500-01

Size / count

0.1 g

Dry ice

No

Hazardous

No

Discontinued

No

Specifications

<ul><li><b style="font-size: small;">Appearance</b><span style="font-size: small;">: </span><span style="font-size: small;">White to off-white amorphous powder, lyophilized</span></li><li><b style="font-size: small;">Activity</b><span style="font-size: small;">: ≥30 U/mg</span></li><li><b style="font-size: small;">DNase</b><span style="font-size: small;">: None detected</span></li><li><b style="font-size: small;">RNase</b><span style="font-size: small;">: None detected</span></li><li><b style="font-size: small;">Nickase</b><span style="font-size: small;">: None detected</span></li></ul>

Applications

<ul><li><b>DNA and RNA Extraction</b></li></ul>Efficiently degrades proteins and nucleases during nucleic acid purification, improving DNA and RNA recovery. <ul><li><b>Molecular Diagnostic Reagents</b></li></ul>Suitable for PCR, qPCR, RT-PCR, and other molecular diagnostic workflows requiring high-purity nucleic acid preparation. <ul><li><b>Genetic Diagnostic Kits</b></li></ul>Widely used in sample pretreatment and nucleic acid extraction processes for genetic testing applications. <ul><li><b>Biopharmaceutical Processing</b></li></ul>Removes protein impurities during the preparation and purification of biological products, including nucleic acid-based therapeutics. <ul><li><b>Tissue and Cell Sample Preparation</b></li></ul>Facilitates the extraction of non-protein components from biological samples through efficient protein digestion.

Concentration

20 mg/mL

Bioactivity

specific activity≥30U/mg, NO Nickase residue, GMP grade

Shipping Conditions

2-8°C

Storage Conditions

-25°C to -15°C