Recombinant Rat PRL Protein (His Tag)

Specifications

Catalog number

PDER100102-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.

Abbreviation

PRL

Synonyms

Lactotrope; LTH; Luteotropic Hormone; Prolactin precursor; PRL; Prolactin

UniProt

P01237

Accession Number

P01237

Expression System

E.coli

Tag

N-His

Sequence

Leu30-Cys266

Field of Research

Metabolism; Developmental Biology; Neuroscience

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26 kDa

Observed Molecular Weight

28 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Rat