- Catalog number
- PDEH101107-01
- Size / count
- 20 µg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1α and SDF1β, which are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. The chemokine CXCL12 [stromal cell-derived factor-1 (SDF-1) ] binds primarily to CXC receptor 4 (CXCR4; CD184) . The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCL12 and CXCR4 that have been widely characterized in peripheral tissues and delineate their main functions in the CNS. Extensive evidence supports CXCL12 as a key regulator for early development of the CNS. In the mature CNS, CXCL12 modulates neurotransmission, neurotoxicity and neuroglial interactions. CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism. Although considered an important factor in normal bone metabolism, recent studies implicate CXCL12 in the pathogenesis of several diseases involving the skeleton, including rheumatoid arthritis and cancers that metastasize to bone. The CXCL12/CXCR4 axis is involved in tumor progression, angiogenesis, metastasis, and survival. Pathologically enhanced CXCL12 signaling may promote the formation of new vessels through recruiting circulating endothelial progenitor cells or directly enhancing the migration/growth of endothelial cells. Therefore, CXCL12 signaling represents an important mechanism that regulates brain tumor angiogenesis/vasculogenesis and may provide potential targets for anti-angiogenic therapy in malignant gliomas.
- Abbreviation
- CXCL12
- Synonyms
- CXCL; SCYB; TPAR; C-X-C Motif Chemokine; Stromal Cell-Derived Factor; hSDF; SDF; CXCL12; IRH; PBSF; SCYB12; SDF1; TLSF; TPAR1; hIRH; C-X-C Motif Chemokine 12; hSDF-1; Intercrine Reduced in Hepatomas; Pre-B Cell Growth-Stimulating Factor; SDF-1; Stromal Cell-Derived Factor 1; SDF1A; SDF1B;12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; Chemokine (C-X-C motif) ligand 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) ; Chemokine CXC motif ligand 12; OTTHUMP00000019491; SDF 1; SDF-1a; SDF-1-alpha (3-67) ; SDF-1b; Stromal cell-derived factor 1 delta; Stromal cell-derived factor 1 gamma; Stromal cell-derived factor 1a; Stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; Tlsfa; TLSF-a; Tlsfb; TLSF-b
- UniProt
- P48061
- Accession Number
- P48061
- Expression System
- E.coli
- Tag
- N-GST
- Sequence
- Lys22-Lys89
- Field of Research
- Neuroscience; Developmental Biology; Cancer; Immunology; Signal Transduction
- Endotoxin
- < 10 EU/mg of the protein as determined by the LAL method
- Purity
- > 90% as determined by reducing SDS-PAGE.
- Bioactivity
- Not validated for activity
- Reconstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
- Shipping Conditions
- This product is provided as lyophilized powder which is shipped with ice packs.
- Storage Conditions
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Calculated Molecular Weight
- 33.5 kDa
- Observed Molecular Weight
- 30 kDa
- Formulation
- Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
- Species
- Human
