Recombinant Human PD-L1 Protein (Sumo Tag)

Specifications

Catalog number

PDEH101109-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Abbreviation

PD-L1

Synonyms

PDL; B7H; PD-L; PD1L; Programmed cell death 1 ligand; PDCD1L; PDCD1LG; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; CD274; B7-H1; B7 homolog 1; CD274 molecule; PDCD1 ligand 1; Programmed death ligand 1; PD1L1; Programmed cell death 1 ligand 1; PDL1; PD-L1; B7-H; B7H1; CD274 molecule; PDCD1L1; PDCD1LG1; B7 H; B7 H1; CD 274; CD274 antigen; MGC142294; MGC142296; OTTHUMP00000021029; PD L1; PDL 1; RGD1566211

UniProt

Q9NZQ7

Accession Number

Q9NZQ7

Expression System

E.coli

Tag

N-Sumo

Sequence

Phe19-Arg238

Field of Research

Cancer; Immunology; Signal Transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.1 kDa

Observed Molecular Weight

43 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human