Matrix Metalloproteinase 3 (MMP-3) Activity Fluorometric Assay Kit

Specifications

Catalog number

E-BC-F061-01

Size / count

48 T (30 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Type

Fluorometric method

Field of Research

Amino Acids and Proteins

Assay Principle

This kit is detected by fluorescence resonance energy transfer (FRET) method. MCA and Dnp are connected to two ends on the natural substrate of MMP-3 enzyme. When MMP-3 protease does not cut the substrate, the two groups are close enough to undergo fluorescence resonance energy transfer, that is, Dnp can quench the fluorescence of MCA, resulting in undetectable fluorescence. When the substrate is cut by MMP-3 protease, the ends and ends of the polypeptide are separated, the two groups are separated, the fluorescence of MCA is no longer extinguished by Dnp, and the fluorescence of MCA can be detected, so that the enzyme activity of MMP-3 protease can be detected very sensitively through fluorescence detection. MCA has a maximum excitation wavelength of 325 nm and a maximum emission wavelength of 393 nm.

Assay Performance Time

50 min

Sample Type

Serum; plasma; animal tissue; cell

Detection Range

0.03-9.66 U/L

Sensitivity

0.03 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

7.5-7.7%

Intra CV

2.0-4.0%

Detection Instrument

Fluorescence Microplate reader