SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified)

Catalog number SPD-C52Hq-100ug

Discontinued

Price for SPD-C52Hq-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike RBD (S477N, E484K), His Tag (SPD-C52Hq) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variant B.1.620, which was found to circulate in Europe and Central Africa.

Specifications

Catalog number: SPD-C52Hq-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Arg 319 - Lys 537
Host: HEK 293
Target: Spike RBD
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike RBD (S477N, E484K), His Tag (Cat. No. SPD-C52Hq) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 26.5 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) is SPD-C52Hq-100ug.

Is SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) in stock?

SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified)?

SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike RBD Protein (S477N, E484K), His Tag (MALS verified) used for?

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.