Human IL-18 Recombinant Rabbit Monoclonal Antibody [PSH02-08]

About this product

Interleukin-18 (IL-18), also known as interferon-gamma inducing factor is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases. IL-18 belongs to the IL-1 superfamily and is produced mainly by macrophages but also other cell types, stimulates various cell types and has pleiotropic functions. IL-18 is a proinflammatory cytokine that facilitates type 1 responses. Together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 in combination with IL12 acts on CD4, CD8 T cells and NK cells to induce IFNγ production, type II interferon that plays an important role in activating the macrophages or other cells. The combination of this IL-18 and IL-12 has been shown to inhibit IL-4 dependent IgE and IgG1 production and enhance IgG2a production in B cells. Importantly, without IL-12 or IL-15, IL-18 does not induce IFNγ production, but plays an important role in the differentiation of naive T cells into Th2 cells and stimulates mast cells and basophils to produce IL-4, IL-13, and chemical mediators such as histamine.

Specifications

Catalog number

HA721767

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Iboctadekin antibody IFN gamma inducing factor antibody IFN-gamma-inducing factor antibody IGIF antibody IL 1 gamma antibody IL 18 antibody IL 1g antibody IL-1 gamma antibody IL-18 antibody IL1 gamma antibody IL18 antibody IL18 protein antibody IL18_HUMAN antibody IL1F4 antibody IL1g antibody IL1gamma antibody ILIF4 antibody Interferon gamma inducing factor antibody Interferon gamma-inducing factor antibody Interleukin 1 gamma antibody Interleukin 18 (interferon-gamma-inducing factor) antibody Interleukin 18 antibody Interleukin-1 gamma antibody Interleukin-18 antibody Interleukin18 antibody MGC12320 antibody

Abbreviation

IL18, IGIF, IL1F4

Swiss Prot

Q14116 Human

Cellular Locus

Cytoplasm, Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within Human IL-18 aa 37-193.

Isotype

IgG

Clone

PSH02-08

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

U-2 OS cell lysate, A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, human liver tissue, human spleen tissue, human tonsil tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:5,000-1:10,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 22 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes