MLH1 Recombinant Rabbit Monoclonal Antibody [PD01-61]

About this product

DNA-mismatch repair (MMR) is an essential process in maintaining genetic stability. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter methylation gene silencing. MLH1 is an integral part of the protein complex responsible for mismatch repair and is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder tissues, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.

Specifications

Catalog number

HA721325

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

COCA 2 antibody COCA2 antibody DNA mismatch repair protein Mlh1 antibody FCC 2 antibody FCC2 antibody hMLH 1 antibody hMLH1 antibody HNPCC 2 antibody HNPCC antibody HNPCC2 antibody MGC5172 antibody MLH 1 antibody MLH1 antibody MLH1_HUMAN antibody MutL homolog 1 (E. coli) antibody MutL homolog 1 antibody MutL homolog 1 colon cancer nonpolyposis type 2 antibody MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody MutL protein homolog 1 antibody MutL, E. coli, homolog of, 1 antibody

Abbreviation

MLH1, COCA2

Swiss Prot

P40692 Human, Q9JK91 Mouse, P97679 Rat

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to Human MLH1 aa 700-756.

Isotype

IgG

Clone

PD01-61

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, HCT 116 cell lysate, A549 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, human appendix tissue, human colon tissue, human B-cell lymphoblastic lymphoma tissue, human NKT cell lymphoma tissue, mouse testis tissue, mouse thymus tissue, human colon carcinoma tissue, rat small intestine tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IHC-P: 1:200-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 85 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes