- Catalog number
- AS05 084A-DL650
- Size / count
- 40 µg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria, The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II, PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts, Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples, Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
- CAS Number
- 9007-83-4
- HS Code
- 38221900
- UniProt
- P83755 , P0C434 , Q14FH6 , Q6YXN7 , P07753 , P14660
- Host
- Rabbit
- Reactivity
- Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp, , Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp, , Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp, , Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrella patens, Synechococcus sp, PCC 7942, Paulinella chromatophora (amoeba), Prochlorococcus sp, (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp, , Zea mays
- Not reactive in
- No confirmed exceptions from predicted reactivity are currently known
- Immunogen
- Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp, , Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp, , Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp, , Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrella patens, Synechococcus sp, PCC 7942, Paulinella chromatophora (amoeba), Prochlorococcus sp, (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp, , Zea mays
- Clonality
- Polyclonal
- Applications
- Immunofluorescence (IF), Western blot (WB)
- Dilution
- To be determined by end user
- Purity
- Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
- Format
- Liquid in PBS pH 7,4.
- Molecular Weight
- 38 | 28-30 kDa
- Additionnal Information
- Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands, Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
- References & Citations
- To be added when available. Antibody released in May 2023.
- Storage Conditions
- Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Shortly spin the tube before use.
- Prediction Reactivity
- Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Cannabis sativa, Galdieria sulphuraria, Lactuca sativa, Lycopersicum esculentum, Medicago sativa, Nannochloropsis sp., Oryza sativa, Ostreococcus sp. Pisum sativum, Porphyridium purpureum, Sesamum indicum, Thalassiosira pseudonana, Zosteria marina, Vitis vinifera cellular [compartment marker] of thylakoid membraneSpecies of your interest not listed? Contact us
