RNF14 Recombinant Rabbit Monoclonal Antibody [JE63-86]

About this product

E3 ubiquitin-protein ligase RNF14 is an enzyme that in humans is encoded by the RNF14 gene. The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. Another function of RNF14 protein relates to its regulation of the inter-relationship between bioenergetic status and inflammation. It influences the expression of mitochondrial and immune-related genes in skeletal muscle including cytokines and interferon regulatory factors.

Specifications

Catalog number

HA722357

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

E3 ubiquitin-protein ligase RNF14 Androgen receptor-associated protein 54 HFB30 RING finger protein 14 RNF14 ARA54 HRIHFB2038

Abbreviation

RNF14, ARA54, HRIHFB2038

Swiss Prot

Q9UBS8 Human, Q9JI90 Mouse,

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide within Human RNF14 aa 341-390 / 474.

Isotype

IgG

Clone

JE63-86

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, SK-Br-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse testis tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 54 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes