CELF1 Recombinant Mouse Monoclonal Antibody [7A1-R]

About this product

Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawl.

Specifications

Catalog number

HA601327

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

50 kDa Nuclear polyadenylated RNA binding protein antibody 50 kDa nuclear polyadenylated RNA-binding protein antibody Bruno like 2 antibody bruno like protein 2 antibody Bruno-like protein 2 antibody BRUNOL 2 antibody BRUNOL2 antibody CELF 1 antibody CELF-1 antibody celf1 antibody CELF1 CUGBP, Elav like family member 1 antibody CELF1_HUMAN antibody CUG BP and ETR 3 like factor 1 antibody CUG BP antibody CUG BP1 antibody CUG RNA binding protein antibody CUG triplet repeat RNA binding protein 1 antibody CUG triplet repeat RNA-binding protein 1 antibody CUG-BP antibody CUG-BP- and ETR-3-like factor 1 antibody CUG-BP1 antibody CUGBP 1 antibody CUGBP and ETR3 like factor 1 antibody CUGBP antibody CUGBP Elav like family member 1 antibody CUGBP Elav-like family member 1 antibody CUGBP1 antibody Cytidine uridine guanosine binding protein 1 antibody Deadenylation factor CUG BP antibody Deadenylation factor CUG-BP antibody Deadenylation factor CUGBP antibody EDEN BP antibody EDEN BP homolog antibody EDEN-BP antibody EDEN-BP homolog antibody embryo deadenylation element binding protein antibody embryo deadenylation element binding protein homolog antibody Embryo deadenylation element-binding protein homolog antibody hNab 50 antibody hNab50 antibody NAB 50 antibody NAB50 antibody NAPOR antibody Nuclear polyadenylated RNA binding protein 50 kD antibody Nuclear polyadenylated RNA binding protein antibody RNA binding protein BRUNOL 2 antibody RNA binding protein BRUNOL2 antibody RNA-binding protein BRUNOL-2 antibody

Abbreviation

CELF-1, CUG-BP1, EDEN-BP homolog, CELF1, BRUNOL2, CUGBP, CUGBP1, NAB50

Swiss Prot

Q92879 Human, P28659 Mouse, Q4QQT3 Rat

Cellular Locus

Cytoplasm. Nucleus.

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within Human CELF1 aa 1-200 / 486.

Isotype

IgG1

Clone

7A1-R

Conjugation

Non-conjugated

Type

Recombinant Mouse monoclonal Antibody

Applications

WB, IF-Cell, IHC-P

Positive Control

HeLa cell lysate, 293T cell lysate, NCI-H226 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, HeLa, human brain tissue, human kidney tissue, mouse brain tissue, mouse hippocampus tissue, rat brain tissue, rat hippocampus tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 52 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes