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Product details
Catalog number 10000-10
Price for 10000-10
Size / count 10 Plates
No wash calcium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular calcium flux. Excitation: 490 nm | Emission: 515 nm ION Biosciences Brilliant Calcium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes intracellular Ca2+ mediated through a wide-variety of plasma membrane and intracellular calcium channels and transporters. The ION Biosciences Brilliant Calcium Assay is also useful for investigating numerous effectors of ion channels and transporters including G protein-coupled receptors, lipid kinases and protein kinases. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Ca2+. For the last 25 years, fluorescence-based measures of Ca2+ flux have brought about the discovery of small-molecule modulators of a host of Ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research. ION Brilliant Calcium Flex provides all the reagents necessary for use as a washed or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Ca2+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.
The catalog number for Brilliant Calcium Flex - No wash calcium assay kit is 10000-10.
Brilliant Calcium Flex - No wash calcium assay kit is available in the following size(s): 10 Plates.
Brilliant Calcium Flex - No wash calcium assay kit is currently made to order / not held in immediate stock. Contact us for lead time.
No wash calcium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular calcium flux. Excitation: 490 nm | Emission: 515 nm ION Biosciences Brilliant Calcium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of c
Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.
A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.
Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.
