BCECF AM - green fluorescent pH indicator

Catalog number 4011E

Price for 4011E

Size / count 20x 50 µg

About this product

BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings. Excitation: 450, 500 nm | Emission: 530 nm | Purity: >95%

Specifications

Catalog number: 4011E
Size / count: 20x 50 µg
Dry ice: No
Hazardous: No
Discontinued: No
Synonyms: 2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein Acetoxymethyl Ester
Weight: 0,5 lbs
Shipping Conditions: Overnight
Shipping Width: 10 in
Shipping Height: 1,5 in
Shipping Length: 12 in

Frequently Asked Questions

What is the catalog number for BCECF AM - green fluorescent pH indicator?

The catalog number for BCECF AM - green fluorescent pH indicator is 4011E.

What sizes are available for BCECF AM - green fluorescent pH indicator?

BCECF AM - green fluorescent pH indicator is available in the following size(s): 20x 50 µg.

Is BCECF AM - green fluorescent pH indicator in stock?

BCECF AM - green fluorescent pH indicator is currently made to order / not held in immediate stock. Contact us for lead time.

What is BCECF AM - green fluorescent pH indicator used for?

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.