- Home
- Shop
- New Categories
- New Blog
- Contact Us
- Cart
- Login
- Register
Select the image to view it full size.
Product details
Catalog number 5100
Price for 5100
Size / count 5 Plates
Mitochondrial membrane potential assay kit that uses JC-10. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays. Healthy: Ex 540 nm / Em 590 nm | Compromised: Ex 490 nm / Em 525 nm ION Vital - MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, the MitoVolt kit provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application. JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.
The catalog number for ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC -10 is 5100.
ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC -10 is available in the following size(s): 5 Plates.
ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC -10 is currently made to order / not held in immediate stock. Contact us for lead time.
ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC -10 is requires dry ice shipping.
Mitochondrial membrane potential assay kit that uses JC-10. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays. Healthy: Ex 540 nm / Em 590 nm | Compromised: Ex 490 nm / Em 525 nm ION Vital - MitoVolt assay kit is the ideal solution for detecting changes in mitochondr
Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.
Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.
Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.
