- Catalog number
- ICH1001-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function. The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58), the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR). Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD2 In Vivo Antibody - Low Endotoxin (G11) recognizes Human CD2. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes
- Product Name Alternative
- Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5
- UniProt
- P06729
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD2
- Isotype
- IgG2a
- Clone
- G11
- Applications
- Western Blot, Functional Assays
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD2 In Vivo Antibody - Low Endotoxin (G11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245