- Catalog number
- ICH1002-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias. The UCHT-1 clone is recognized in the human leukocyte differentiation antigen workshop III 471.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)
- Product Name Alternative
- CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain
- UniProt
- P07766
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD3
- Isotype
- IgG1
- Clone
- UCHT-1
- Applications
- Flow Cytometry, Immunoprecipitation, Western Blot, IHC (Frozen), CyTOF, Functional Assays
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- ≥ 2.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin.
Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247
