- Catalog number
- ICH1006-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Anti-CD11a may be used to study a variety of biological phenomena related to the function of cell adhesion molecules. This antibody may be used to study transplantation rejections and to assist in characterizing lymphomas and leukemias.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD11a In Vivo Antibody - Low Endotoxin (38) recognizes Human CD11a. Anti-Human CD11a (Anti-LFA-1a) recognizes the (Mr 175 kDa) a chain of the leukocyte function associated antigen-1 (LFA-1). The leukocyte integrin LFA-1 is a member of a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. LFA-1A binds to domain 1 of an intercellular adhesion molecule (ICAM) on target tissues.
- Product Name Alternative
- LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL
- UniProt
- P20701
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD11a
- Isotype
- IgG2a
- Clone
- 38
- Applications
- Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD11a In Vivo Antibody - Low Endotoxin (38) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The CD11a antigen is present on most leukocytes and plays a vital role in cellular immune responses which require adhesion. Lymphocyte activation via the T-cell antigen receptor (TCR) up-regulates LFA-1 function. The absence of LFA-1A or B may induce the disease state known as leukocyte adhesion deficiency (LAD). Tumor cells which do not express LFA-1 may have avoided cellular immune-responses such as those carried out by natural killer cells (NK).
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245
