Bulk Anti-Human CD16 (3G8) Antibody

Catalog number ICH1010-25mg

Price for ICH1010-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1010-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.
CAS Number: 9007-83-4
Specifications: Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16
Product Name Alternative: FCGR3A, Fc-gamma RIII, FcRIII
UniProt: P08637
Host: Mouse
Species Reactivity: Human
Target: CD16
Isotype: IgG1 Kappa
Clone: 3G8
Applications: IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: ≥ 2.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.
Antigen Distribution: CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247

Frequently Asked Questions

What is the catalog number for Bulk Anti-Human CD16 (3G8) Antibody?

The catalog number for Bulk Anti-Human CD16 (3G8) Antibody is ICH1010-25mg.

What sizes are available for Bulk Anti-Human CD16 (3G8) Antibody?

Bulk Anti-Human CD16 (3G8) Antibody is available in the following size(s): 25 mg.

Is Bulk Anti-Human CD16 (3G8) Antibody in stock?

Bulk Anti-Human CD16 (3G8) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

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