- Catalog number
- ICH1012-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The CD20 antigen, also called MS4A1, B1 and Bp35, is a non-glycosylated, transmembrane spanning, phosphoprotein. The CD20 protein is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells, and is synchronous with the expression of surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset. CD20 has been shown to be heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complex with MHC class I and II, CD53, CD81, and CD82. Extensive CD20 cross-linking may also influence cell death by apoptosis. Therapy with antibodies against CD20 is a widely used regimen in the treatment of all B cell lymphomas and leukemias.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD20 In Vivo Antibody - Low Endotoxin (2H7) recognizes an epitope on Human CD20
- Product Name Alternative
- B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
- UniProt
- P11836
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD20
- Isotype
- IgG2b Kappa
- Clone
- 2H7
- Applications
- Flow Cytometry, CyTOF, IHC (Frozen), Immunoprecipitation
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD20 In Vivo Antibody - Low Endotoxin (2H7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2b-Isotype-Control-for-In-Vivo-Low-Endotoxin-MPC-11-ICH2250
