Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin

Catalog number ICH1027UL-25mg

Price for ICH1027UL-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1027UL-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.
CAS Number: 9007-83-4
Specifications: Mouse anti-Human Inter-Cellular Adhesion Molecule 1 (ICAM-1) (Clone 15.2) - Ultra-Low Endotoxin recognizes an epitope on Human ICAM-1
Product Name Alternative: Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, CD54
UniProt: P05362
Host: Mouse
Species Reactivity: Human, Mouse, Rat
Target: ICAM-1
Isotype: IgG1
Clone: 15.2
Applications: IHC (FFPE), Flow Cytometry, Immunoprecipitation, Western Blot
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: 1.0 - 5.0 mg/mL
Endotoxin: ≤ 0.5 EU/mg as determined by the LAL method
Purity: >98% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria.
Aggregation: Aggregation level ≤ 1%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247

Frequently Asked Questions

What is the catalog number for Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin?

The catalog number for Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin is ICH1027UL-25mg.

What sizes are available for Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin?

Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin is available in the following size(s): 25 mg.

Is Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin in stock?

Anti-ICAM-1 (15.2) In Vivo Antibody - Ultra-Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.