- Catalog number
- ICH1028-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interferon-gamma (IFN-gamma) exerts its biologic effects by interacting with a single high affinity receptor, IFN-gamma receptor. This receptor is present on almost all cell types except mature erythrocytes. The IFN-gamma receptor consists of two polypeptide chains alpha and beta and requires association of JAK1, JAK2 and Stat1 for IFN-gamma signal transduction. In signal transduction, IFN-gamma induces tyrosine phosphorylation of IFN-gamma receptor alpha (IFNGR1) leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-gamma induced gene transcription. The IFN-gamma receptor is structurally related to the recently cloned IL-10 receptor. GIR 208 binds IFNGR1 and blocks binding of IFN-gamma to the receptor.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD119 In Vivo Antibody - Low Endotoxin (GIR 208) recognizes Human IFN gamma R alpha
- Product Name Alternative
- Interferon gamma receptor 1, IFN-gamma receptor 1, IFN-gamma-R1, CDw119, Interferon gamma receptor alpha-chain2, IFN-gamma-R-alpha
- UniProt
- P15260
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD119
- Isotype
- IgG1
- Clone
- GIR 208
- Applications
- Immunoprecipitation, Western Blot, ELISA, Neutralization, Flow Cytometry
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD119 In Vivo Antibody - Low Endotoxin (GIR 208) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The IFN-gamma receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247
