Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin

Catalog number ICH1036-100mg

Price for ICH1036-100mg

Size / count 100 mg

Specifications

Catalog number: ICH1036-100mg
Size / count: 100 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: 4-1BB (CD137) is a costimulatory molecule expressed on the surface of activated T cells and plays a role in T-cell-mediated proliferative response. 4-1BB signal regulates CD28 co-stimulation in the targeted subset cells to favor Th1 development and maintain long-term cell growth. 4-1BB is also expressed on dendritic cells (DCs) and controls the duration, DC-T interaction, and, therefore, immunogenicity. Clone 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.
CAS Number: 9007-83-4
Specifications: Anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) recognizes an epitope on Mouse 4-1BB
Product Name Alternative: 4-1BB ligand receptor, T-cell antigen 4-1BB, Tnfrsf9, Ila, Ly63, CD137
UniProt: P20334
Host: Rat
Species Reactivity: Mouse
Target: 4-1BB
Isotype: IgG2a Kappa
Clone: 3H3
Applications: Western Blot, Functional Assays, ELISA, Stimulation, Immunoprecipitation
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 1.0 - 5.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: Activated T cells and B cells
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

Frequently Asked Questions

What is the catalog number for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is ICH1036-100mg.

What sizes are available for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin?

Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is available in the following size(s): 100 mg.

Is Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

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