Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin

Catalog number ICH1036-25mg

Price for ICH1036-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1036-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: 4-1BB (CD137) is a costimulatory molecule expressed on the surface of activated T cells and plays a role in T-cell-mediated proliferative response. 4-1BB signal regulates CD28 co-stimulation in the targeted subset cells to favor Th1 development and maintain long-term cell growth. 4-1BB is also expressed on dendritic cells (DCs) and controls the duration, DC-T interaction, and, therefore, immunogenicity. Clone 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.
CAS Number: 9007-83-4
Specifications: Anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) recognizes an epitope on Mouse 4-1BB
Product Name Alternative: 4-1BB ligand receptor, T-cell antigen 4-1BB, Tnfrsf9, Ila, Ly63, CD137
UniProt: P20334
Host: Rat
Species Reactivity: Mouse
Target: 4-1BB
Isotype: IgG2a Kappa
Clone: 3H3
Applications: Western Blot, Functional Assays, ELISA, Stimulation, Immunoprecipitation
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 1.0 - 5.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: Activated T cells and B cells
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

Frequently Asked Questions

What is the catalog number for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is ICH1036-25mg.

What sizes are available for Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin?

Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is available in the following size(s): 25 mg.

Is Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse 4-1BB (3H3) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.