- Catalog number
- ICH1037-25mg
- Size / count
- 25 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80
- Product Name Alternative
- Activation B7-1 antigen, B7
- UniProt
- Q00609
- Host
- Armenian Hamster
- Species Reactivity
- Mouse
- Target
- CD80
- Isotype
- IgG
- Clone
- 16-10A1
- Applications
- Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251