Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin

Catalog number ICH1037-50mg

Price for ICH1037-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1037-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.
CAS Number: 9007-83-4
Specifications: Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80
Product Name Alternative: Activation B7-1 antigen, B7
UniProt: Q00609
Host: Armenian Hamster
Species Reactivity: Mouse
Target: CD80
Isotype: IgG
Clone: 16-10A1
Applications: Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: 1.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

Frequently Asked Questions

What is the catalog number for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is ICH1037-50mg.

What sizes are available for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin?

Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.