Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin

Catalog number ICH1037-5mg

Price for ICH1037-5mg

Size / count 5 mg

Specifications

Catalog number: ICH1037-5mg
Size / count: 5 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD80 (B7-1) is a type I integral membrane protein related very closely to CD86 (B7-2). A main function of CD80 is the costimulation of T-cells through ligation of CD28 and CD152 (CTLA-4). T-cells activate with binding of CD28 to CD80 and binding of CD80 to CTLA-4 results in inhibition of T-cells. The clone 16-10A1 has the ability to block the costimulatory activity of CD80 while exhibiting no effect on the activity of CD86.
CAS Number: 9007-83-4
Specifications: Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) recognizes an epitope on Mouse CD80
Product Name Alternative: Activation B7-1 antigen, B7
UniProt: Q00609
Host: Armenian Hamster
Species Reactivity: Mouse
Target: CD80
Isotype: IgG
Clone: 16-10A1
Applications: Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen)
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: 1.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD80 In Vivo Antibody - Low Endotoxin (16-10A1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: Mouse CD80 is expressed on Activated B-Cells, Activated T-Cells, macrophages, and dendritic cells.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

Frequently Asked Questions

What is the catalog number for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is ICH1037-5mg.

What sizes are available for Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin?

Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is available in the following size(s): 5 mg.

Is Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD80 (16-10A1) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

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