Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin

Catalog number ICH1040-50mg

Price for ICH1040-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1040-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex.
CAS Number: 9007-83-4
Specifications: Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3ε
Product Name Alternative: T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
UniProt: P22646
Host: Syrian Hamster
Species Reactivity: Mouse
Target: CD3e
Clone: 500A2
Applications: Functional Assays, Flow Cytometry, IHC (Frozen)
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: 1.0 mg/mL
Endotoxin: <1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specific applications.

Frequently Asked Questions

What is the catalog number for Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin is ICH1040-50mg.

What sizes are available for Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin?

Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

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