Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin

Catalog number ICH1041-50mg

Price for ICH1041-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1041-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
CAS Number: 9007-83-4
Specifications: Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e
Product Name Alternative: T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e
UniProt: P22646
Host: Armenian Hamster
Species Reactivity: Mouse
Target: CD3e
Isotype: IgG
Clone: 145-2C11
Applications: IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: 1.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD3e In Vivo Antibody - Low Endotoxin (145-2C11) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

Frequently Asked Questions

What is the catalog number for Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin is ICH1041-50mg.

What sizes are available for Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin?

Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD3e (145-2C11) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.