Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

Catalog number ICH1042-50mg

Price for ICH1042-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1042-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
CAS Number: 9007-83-4
Specifications: Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Product Name Alternative: T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
UniProt: P06332
Host: Rat
Species Reactivity: Mouse
Target: CD4
Isotype: IgG2b Kappa
Clone: GK1.5
Applications: Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: ≥ 2.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is ICH1042-50mg.

What sizes are available for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin?

Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.