Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

Catalog number ICH1042-5mg

Price for ICH1042-5mg

Size / count 5 mg

Specifications

Catalog number: ICH1042-5mg
Size / count: 5 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
CAS Number: 9007-83-4
Specifications: Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Product Name Alternative: T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
UniProt: P06332
Host: Rat
Species Reactivity: Mouse
Target: CD4
Isotype: IgG2b Kappa
Clone: GK1.5
Applications: Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: ≥ 2.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is ICH1042-5mg.

What sizes are available for Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin?

Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is available in the following size(s): 5 mg.

Is Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.