Anti-Mouse CD8 (YTS-169) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1043-100mg

Size / count

100 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) recognizes an epitope on Mouse CD8

Host

Rat

Species Reactivity

Mouse

Target

CD8

Isotype

IgG2b

Clone

YTS-169

Applications

IHC (Frozen), In vivo Depletion, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

<0.5 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections but the concentration is assay dependent. Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243