Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin

Catalog number ICH1044-50mg

Price for ICH1044-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1044-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.
CAS Number: 9007-83-4
Specifications: Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.
Product Name Alternative: CD8 alpha chain, Lyt-2, Lyt2
UniProt: P01731
Host: Rat
Species Reactivity: Mouse
Target: CD8a
Isotype: IgG2a Kappa
Clone: 53-6.7
Applications: Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen)
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 1.0 - 5.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD8a In Vivo Antibody - Low Endotoxin (53-6.7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Immunoprecipitation: Rat Anti-Mouse CD8a (Clone 53-6.7) precipitates 44 kDa and 33 kDa proteins. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin is ICH1044-50mg.

What sizes are available for Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin?

Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse CD8a (53-6.7) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.