Anti-Mouse CD8a (2.43) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1045-25mg

Size / count

25 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) recognizes an epitope on Mouse CD8a

Product Name Alternative

CD8 alpha chain, Lyt-2, Lyt2

UniProt

P01731

Host

Rat

Species Reactivity

Mouse

Target

CD8a

Isotype

IgG2b

Clone

2.43

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, Immunofluorescence, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8a In Vivo Antibody - Low Endotoxin (2.43) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

CD8a (Ly 2.2) is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic-cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243