Anti-Mouse CD8 Beta (53-5.8) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1046-25mg

Size / count

25 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.

CAS Number

9007-83-4

Specifications

Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) recognizes an epitope on mouse CD8b.2 and antibody clone 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1

Product Name Alternative

Cd8b, Lymphocyte antigen 3, T-cell membrane glycoprotein Ly-3, T-cell surface glycoprotein Lyt-3, Cd8b1, Ly-3, Lyt-3, Lyt3

UniProt

P10300

Host

Rat

Species Reactivity

Mouse

Target

CD8 beta

Isotype

IgG1

Clone

53-5.8

Applications

Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD8 beta In Vivo Antibody - Low Endotoxin (53-5.8) is stable for at least one month when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Majority of thymocytes, cytotoxic T cell subsets

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246