Anti-Mouse CD11a (I21/7) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1048-100mg

Size / count

100 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Anti-CD11a In Vivo Antibody - Low Endotoxin (I21/7) recognizes the alpha subunit of the mouse leukocyte function associated antigen-1 (LFA-1). CD11a/CD18ALB2 leukocyte integrin is a member of a family of heterodimeric receptors which mediate cellular adhesion reactions

Product Name Alternative

LFA-1 alpha, LFA-1A, Integrin alpha-L, ITGAL, Ly-15

UniProt

P24063

Host

Rat

Species Reactivity

Mouse

Target

CD11a

Isotype

IgG2a

Clone

I21/7

Applications

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-CD11a In Vivo Antibody - Low Endotoxin (I21/7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

The CD11a LFA-1 antigen is present on >97% thymocytes, >95% blood lymphocytes, 79% bone marrow cells and certain lymphoma and macrophage-like cell lines.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244