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Product details

Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin

Catalog number ICH1049-100mg

Price for ICH1049-100mg

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Size / count 100 mg

Specifications

Catalog number
ICH1049-100mg
Size / count
100 mg
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Specifications
Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) recognizes the (Mr 170 kD) alpha m subunit of Mac-1 (CD11b/CD18, alpha m beta 2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).
Product Name Alternative
Integrin alpha-M, Itgam, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1
UniProt
P05555
Host
Rat
Species Reactivity
Mouse
Target
CD11b
Isotype
IgG2b
Clone
M1/70
Applications
Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), CyTOF, Immunocytochemistry
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
0.5 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Functional Assay: Activation of mouse peritoneal macrophages Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is ICH1049-100mg.

What sizes are available for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin?

Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is available in the following size(s): 100 mg.

Is Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

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