- Catalog number
- ICH1049-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) recognizes the (Mr 170 kD) alpha m subunit of Mac-1 (CD11b/CD18, alpha m beta 2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).
- Product Name Alternative
- Integrin alpha-M, Itgam, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1
- UniProt
- P05555
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- CD11b
- Isotype
- IgG2b
- Clone
- M1/70
- Applications
- Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), CyTOF, Immunocytochemistry
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- 0.5 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Functional Assay: Activation of mouse peritoneal macrophages
Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243