Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin

Catalog number ICH1049-5mg

Price for ICH1049-5mg

Size / count 5 mg

Specifications

Catalog number: ICH1049-5mg
Size / count: 5 mg
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Specifications: Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) recognizes the (Mr 170 kD) alpha m subunit of Mac-1 (CD11b/CD18, alpha m beta 2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).
Product Name Alternative: Integrin alpha-M, Itgam, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1
UniProt: P05555
Host: Rat
Species Reactivity: Mouse
Target: CD11b
Isotype: IgG2b
Clone: M1/70
Applications: Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), CyTOF, Immunocytochemistry
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 0.5 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Functional Assay: Activation of mouse peritoneal macrophages Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is ICH1049-5mg.

What sizes are available for Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin?

Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is available in the following size(s): 5 mg.

Is Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse CD11b (M1/70) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

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