Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin

Catalog number ICH1056-50mg

Price for ICH1056-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1056-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
CAS Number: 9007-83-4
Specifications: Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Product Name Alternative: Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
UniProt: P08508
Host: Rat
Species Reactivity: Mouse
Target: CD32/CD16
Isotype: IgG2b
Clone: 2.4G2
Applications: Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 0.5 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin is ICH1056-50mg.

What sizes are available for Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin?

Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin is available in the following size(s): 50 mg.

Is Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.