Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin

Catalog number ICH1059-25mg

Price for ICH1059-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1059-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.
CAS Number: 9007-83-4
Specifications: Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) recognizes an epitope on Mouse ICAM-1
Product Name Alternative: Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10
UniProt: P13597
Host: Rat
Species Reactivity: Mouse
Target: CD54
Isotype: IgG2b Kappa
Clone: YN1/1.7.4
Applications: Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 1.0 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution: The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin is ICH1059-25mg.

What sizes are available for Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin?

Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin is available in the following size(s): 25 mg.

Is Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin in stock?

Anti-Mouse CD54 (YN1/1.7.4) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.