- Catalog number
- ICH1060-25mg
- Size / count
- 25 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, and LAM-1. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) recognizes an epitope on Mouse CD62L
- Product Name Alternative
- L-selectin, Sell, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1
Lymph node homing receptor1, Lymphocyte antigen 221,
Ly-221, Lymphocyte surface MEL-14 antigen, Lnhr, Ly-22, Ly22
- UniProt
- P18337
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- CD62L
- Isotype
- IgG2a
- Clone
- MEL-14
- Applications
- Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen), CyTOF
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244