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Product details

Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin

Catalog number ICH1060-5mg

Price for ICH1060-5mg

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Size / count 5 mg

Specifications

Catalog number
ICH1060-5mg
Size / count
5 mg
Dry ice
No
Hazardous
No
Discontinued
No
Background
CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, and LAM-1. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
CAS Number
9007-83-4
Specifications
Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) recognizes an epitope on Mouse CD62L
Product Name Alternative
L-selectin, Sell, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1 Lymph node homing receptor1, Lymphocyte antigen 221, Ly-221, Lymphocyte surface MEL-14 antigen, Lnhr, Ly-22, Ly22
UniProt
P18337
Host
Rat
Species Reactivity
Mouse
Target
CD62L
Isotype
IgG2a
Clone
MEL-14
Applications
Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, IHC (Frozen), CyTOF
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
1.0 - 5.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions
Blue ice
Storage Conditions
Anti-CD62L In Vivo Antibody - Low Endotoxin (MEL-14) is stable for at least six (6) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

Frequently Asked Questions

What is the catalog number for Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin is ICH1060-5mg.

What sizes are available for Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin?

Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin is available in the following size(s): 5 mg.

Is Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD62L (MEL-14) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

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