Anti-Mouse CD90 (HK2.1) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1065-25mg

Size / count

25 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Rat anti-Mouse CD90 (Clone HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. This antibody is reported to stimulate mouse T-cell proliferation.

Product Name Alternative

Thy-1, Thy1

UniProt

P01831

Host

Rat

Species Reactivity

Mouse

Target

CD90

Isotype

IgG2c

Clone

HK2.1

Applications

Western Blot, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Functional Assay: Anti-Mouse CD90 (Thy-1) (clone HK2.1) has been implicated in activation of T-cells. Each investigator should determine their own optimal working dilution for specific applications.