- Catalog number
- ICH1073-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD40) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) recognizes an epitope on Mouse CD40
- UniProt
- P27512
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- CD40
- Isotype
- IgG2a
- Clone
- FGK4.5
- Applications
- Western Blot, Functional Assays, Flow Cytometry
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- 1.0 - 5.0 mg/mL
- Endotoxin
- <0.5 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-CD40 In Vivo Antibody - Low Endotoxin (FGK4.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added
- Antigen Distribution
- B cells, basal epithelial cells, macrophages, follicular dendritic cells, and endothelial cells
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
