- Catalog number
- ICH1099-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Mouse IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine.
- CAS Number
- 9007-83-4
- Specifications
- Anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) recognizes Mouse IL-1A
- Product Name Alternative
- Interleukin-1 alpha, IL-1a
- UniProt
- P01582
- Host
- Armenian Hamster
- Species Reactivity
- Mouse
- Target
- IL-1a
- Isotype
- IgG1
- Clone
- ALF-161
- Applications
- Western Blot, ELISA, ELISPOT, Neutralization, Immunoprecipitation, Flow Cytometry (Intracellular)
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1.0 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251