- Catalog number
- ICH1101-25mg
- Size / count
- 25 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies
- CAS Number
- 9007-83-4
- Specifications
- Anti-ICAM-1 In Vivo Antibody - Low Endotoxin (BE29G1) recognizes the (Mr 95 kD) cell surface glycoprotein known as intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).
- Product Name Alternative
- Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10, CD54
- UniProt
- P13597
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- ICAM-1
- Isotype
- IgG2a
- Clone
- BE29G1
- Applications
- Immunoprecipitation, Western Blot, Blocking
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- 0.5 mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- Anti-ICAM-1 In Vivo Antibody - Low Endotoxin (BE29G1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
- Antigen Distribution
- The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Blocking: This antibody blocks the binding LFA-1 (CD11a/CD18) to ICAM-1. The antibody is suitable for studies of inflammation, lymphocyte differentiation, and lymphocyte activation.
Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244
