Anti-Mouse Ly-6C (HK1.4) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1111-50mg

Size / count

50 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) recognizes an epitope on Mouse Ly-6C

Product Name Alternative

Lymphocyte antigen 6 complex, locus C

UniProt

P0CW02

Host

Rat

Species Reactivity

Mouse

Target

Ly-6C

Isotype

IgG2c

Clone

HK1.4

Applications

Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

0.5 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

Anti-Ly-6C In Vivo Antibody - Low Endotoxin (HK1.4) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Formulation

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

Antigen Distribution

The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5

Aggregation

Aggregation level ≤ 5%

Applications Notes

Immunoprecipitation: 1–2 μg per 100–500 μg of total protein (1 ml of cell lysate). Each investigator should determine their own optimal working dilution for specific applications.