Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin

Catalog number ICH1130-25mg

Price for ICH1130-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1130-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.
CAS Number: 9007-83-4
Specifications: Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.
Product Name Alternative: Leukocyte common antigen, L-CA, T200, CD45, Receptor-type tyrosine-protein phosphatase C, PTPRC, B220
UniProt: P08575
Host: Rat
Species Reactivity: Mouse, Human
Target: CD45R
Isotype: IgG2a
Clone: RA3-6B2
Applications: Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA
Purification Method: This monoclonal antibody was purified using Protein G
Concentration: 0.5 mg/mL
Endotoxin: ≤ 1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE and HPLC
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Formulation: No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Antigen Distribution: The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .
Aggregation: Aggregation level ≤ 5%
Applications Notes: Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244

Frequently Asked Questions

What is the catalog number for Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin?

The catalog number for Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin is ICH1130-25mg.

What sizes are available for Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin?

Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin is available in the following size(s): 25 mg.

Is Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin in stock?

Anti-CD45R (RA3-6B2) In Vivo Antibody - Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

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